Contact an Account ManagerSeparation of dyes
Summary
Type: HPLC
Application #: 124680
Catalog#: 760479.30 - MN HPLC Column, Nucleodur, PolarTec, 110A, 3um, 3x250mm
Detection:
Conditions: Eluent A: methanol
Eluent B: 25 mmol/L KH2PO4, pH 7.0
Gradient: 39 % B for 3 min , in 4 min to 30 % B , in 8 min to 10 % B, to 0 % B in 10 min, hold for 5 min
Flow rate: 0.7 mL/min
Temperature: 50 °C
Injection volume: 5.0 µL
Authors: HPLC department
Source: Macherey-Nagel, Germany 2011